Sheeko-sheeko (SARS-Cov-2) BILAASH MADAXWEYNAHA MADAXWEYNAHA PCR

Sharraxaadda Gaaban:

Nadiifin 500190 Cayimid 96 Imtixaanno / Sanduuq
Mabda'a ogaanshaha Tuubbo Tijaabin Nasal / nasopharnngngngeal Swab
Adeegsiga loogu talagalay Tan waxaa loogu talagalay in loo isticmaalo ogaanshaha aqoonta ee SARS-Covel rna viral-ka laga soo saaray suuxshiyaha nasopharngeal, xaako iyo balaastigga soo saarista soo saarista ee loo yaqaan 'FDA / CEF's' iyo aaladaha loo yaqaan 'PCR' ee kor lagu soo sheegay.

Qalabka waxaa loogu talagalay in lagu isticmaalo shaqaale tababbaran oo sheybaarka ah

 


Si faahfaafo

Calaamadaha wax soo saarka

Qalabkan xasaasiga ah ee loo yaqaan 'PCR' ee loo yaqaan 'PCR' ee loo yaqaan 'PCR' ee loo yaqaan 'PCR' ee loo yaqaan 'PCR-ga' PCR-ga Qalabka ayaa lagu qaadi karaa laguna keydin karaa heerkulka qolka oo uu deggan yahay hal sano. Tuub kasta oo ka mid ah pepe of preaterx waxaa ku jira dhammaan reages-ka looga baahan yahay cod-bixinta PCR, oo ay ku jiraan dib-u-celin-wax-ka-rogida, taq polymarese, polympers, baaritaanno, iyo substrates dntps. Kaliya waxay u baahan tahay ku dar 13-ka biyo fog iyo 5ul ah oo la soo saaray Template RNA, ka dib waa la socodsiin karaa oo lagu xoojiyaa astaanta PCR-ga.

Mashiinka QPR waa inuu buuxiyaa shuruudaha soo socda:
1. Ku habboon 8 strip pcr tuubada tubada 0.2 ml
2. Waxay leeyihiin in kabadan afar kanal oo loo yaqaan ':

Kanaal

DIIWAANKA (NM)

Ka soo baxa (nm)

Dyes pre-calated

1.

470

525

FAM, SYBR Green i

2

523

564

Vic, Hex, TET, Joe

3.

571

621

Rox, Texas-casaan

4

630

670

Cy5

PCR-MARKA:
Nidaamka PCR-ka ee 750000al-PCR, Biorad CF96, Icycler IQ-da-ka dib-u-habeynta PCR-ka ee PCR, StreetGene Mx3000P, Mx3005p

Dhibaatada silsiladda qabow ee taraafikada qabow ee 'coronavirus' ee coronavirus 'ee coronavic acid'
Marka dib-u-celinta ogaanshaha caadiga ah ee loo yaqaan 'Nucleic acid' oo caadi ah, (-20 ± 5) ℃ Kaydinta silsiladda qabow iyo gaadiid ayaa loo baahan yahay si loo hubiyo in biolem-ka ee enzyme ee relates-ka ay shaqeeyaan. Si loo hubiyo in heerkulku gaaro heerka, dhowr kiilogaraam oo baraf qalalan ayaa looga baahan yahay sanduuq kasta oo tijaabada ah ee tijaabada aashitada nucleic acid ah oo ka dambaysa in ka yar 50g, laakiin waxay socon kartaa oo keliya laba ama saddex maalmood. Marka laga eego aragtida warshadaha ee warshadaha, culeyska dhabta ah ee ka-hortagga ay soo saareen soosaarayaasha ayaa ka yar 10% (ama aad uga yar tahay qiimahan) weelkan) weelkan Miisaanka badankood wuxuu ka yimaadaa barafka qallalan, xirmooyinka barafka iyo sanaadiiqda ee barafka, si kharashka gaadiidku aad u sarreeyo.

Bishii Maarso 2020, doopid-19 wuxuu bilaabay inuu ka baxo qiyaas baahsan oo dibedda ah, oo baahida loo qabo in la ogaado coronavirus-ka cusub ee coronavirus nucleic si aad u kordhay. In kasta oo ay tahay qiimaha sare ee dhoofinta dadka ku qoran silsiladaha qabow, inta badan soosaarayaasha ayaa wali aqbali kara sababta oo ah tirada badan iyo faa iido badan darteed.

Si kastaba ha noqotee, horumarinta siyaasadaha dhoofinta qaranka ee wax soo saarka ka-hortagga faafa, iyo sidoo kale kor u qaadida xakameynta qaran ee qulqulka dadka iyo saadka, oo sababay in la helo dhibaatooyin waqti ah oo ay sababaan gaadiidka. Waqti dheeri ah oo gaadiid ah (Gaadiidka Waqtiga Qiyaastii nus-ka badh bishii waa mid aad u badan), waxay horseedaa in wax soo saarka soo noqnoqda ee soo noqnoqda markii sheygu gaaro macmiilka. Tani waxay dhibaataysan inta badan realgic-ka dhoofinta nucleic acid ah ee dhoofinta.

Teknolojiyad Lyophililismay oo loogu talagalay PCRENT ee PCRENT ayaa ka caawisay gaadiidka ku saabsan soo-saarista sheeko-sheeko ee coronavirus-ka aashitada nucleic ee adduunka oo dhan

Reagys PCR-ga ayaa lagu qaadi karaa oo lagu keydin karaa heerkulka qolka, oo aan yarayn karin oo keliya qiimaha gaadiidka, laakiin sidoo kale iska ilaali dhibaatooyinka tayada ee ay sababaan geedi socodka gaadiidka. Sidaa darteed, waa in laga fiirsadaa reagenent waa habka ugu wanaagsan ee lagu xalliyo dhibaatada gaadiidka dhoofinta.

Lyphoplization-ka waxay ku lug leedahay in xalka lagu habeeyo xaalad adag, ka dibna ku qudhxidho oo u kala soocaan uumiga biyaha ka hooseeya xaalad vacuum ah. Soute-ka qalalan ayaa ku jira weelka oo leh isku xirnaan iyo waxqabad isku mid ah. Marka la barbar dhigo reagents-ka dareeraha caadiga ah, oo ah xarkaha buuxa ee 'Coronavirus' ee 'Corophirus' ee Coroplirus-ka Nucleic acid ee ay soo saartay iyada oo xaddidaadda bio ay leedahay astaamaha soo socda:

Deganaanshaha kuleylka aad u xoog badan:Waxay ku daweyn kartaa daweyn ahaanta 56 ℃ 60 maalmood, iyo horumarka maskaxda iyo waxqabadka ee dib-u-habeynta ah wali isma beddelaan.
Kaydinta heerkulka caadiga ah iyo gaadiidka:Looma baahna silsilad qabow, looma baahna in lagu keydiyo heerkulka hoose ka hor aan la xallin ka hor, si buuxda u sii daaya meesha lagu keydiyo qabowga.
Isticmaalka Diyaar-u-ah:Lyoficiddiisa dhammaan qaybaha, looma baahna qaabeynta nidaamka, oo ka fogaanaya luminta qaybaha leh viscosity-ka sare sida enzyme.
Bartilmaameedyada badan ee hal tubbo:Bartilmaameedka ogaanshaha ayaa daboolaya buuraha 'Coronavirus' ee Cronavirus Oronavirus Orf1b hiddo-wadaha, n hiddo-wadaha si looga fogaado taranka jikada. Si loo yareeyo hiddaha beenta ah ee beenta ah, ee 'RNASE PNASE PNEASE PNEASE' waxaa loo isticmaalaa sidii xakameyn gudaha ah, si ay ula kulmaan baahiyaha caafimaad ee saamaynta tayada tayada saamiga.


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